Phil Mickelson and Ian Poulter are among 11 LIV Golf players who have taken legal action against the PGA Tour to challenge their suspensions.
The PGA Tour has suspended all of its members who signed up for the controversial Saudi-backed LIV series, which is in its inaugural season.
2020 US Open champion Bryson DeChambeau has also put his name to the anti-trust lawsuit.
The group claims the PGA Tour is trying to harm their careers.
“The Tour’s conduct serves no purpose other than to cause harm to players and prevent the entry of the first significant competitive threat the Tour has faced in decades,” the lawsuit states.
“The purpose of this action is to overturn anti-competitive PGA Tour rules and practices that prevent these independent golfers from playing when and where they choose.”
In a letter responding to the lawsuit that was sent to PGA Tour members, commissioner Jay Monahan said the organization would not back down.
“These suspended players – who are now employees of the Saudi Golf League – left the Tour and now want to come back,” he said.
“It’s an attempt to use the Tour platform to promote yourself and take advantage of your benefits and efforts.
“Allowing re-entry into our events compromises the Tour and the competition, to the detriment of our organization, our players, our partners and our supporters.
“The lawsuit they filed somehow expects us to believe otherwise, which is why we intend to make our case clearly and vigorously.”
The PGA Tour suspended those who joined the breakaway series in June, although those players were still able to play in the subsequent US Open and last month’s Open Championship.
Six-time major winner Mickelson and other major champions including Dustin Johnson, Brooks Koepka, Patrick Reed and Sergio Garcia were among those suspended.
Poulter was also suspended by the Europe-based DP World Tour after following the PGA Tour’s lead.
The move prompted 16 players to threaten legal action if the bans were upheld and Poulter was able to play at the Scottish Open after his ban was lifted pending a full appeal.
Carlos Ortiz, Peter Uihlein, Jason Kokrak, Pat Perez, Abraham Ancer, Talor Gooch, Hudson Swafford and Matt Jones are the other plaintiffs in the lawsuit against the PGA Tour.
Gooch, Swafford and Jones are asking for temporary relief to allow them to compete in the FedEx Cup play-offs, which begin next week.