The PGA Tour is raising the prices of 12 events to match those of LIV Golf, but the tour chief said players who have already joined the breakaway league will not be welcome.
Commissioner Jay Monahan announced on Wednesday that 12 PGA events will now have an average purse of $20m (£16.9m).
The best players will play in the 12 and 20 events in total, throughout the season.
The changes come after Rory McIlroy and Tiger Woods met other top Tour players to discuss the threat of LIV Golf.
A number of top players have left established tours for the new Saudi-backed eight-event series, which has a £200million prize pool.
Big winners Brooks Koepka, Phil Mickelson, Bryson DeChambeau and Dustin Johnson are among those who have joined, and the PGA has responded with indefinite bans.
When asked if those who left would be welcome if they were attracted to the changes to the PGA Tour, Monahan replied: “No. They joined the LIV Golf Series and they made that commitment.
“Most of them they made multi-year commitments. As I’ve been clear throughout, every player has a choice, and I respect their choice, but they made it. We made ours. .”
Reigning Open and Players Championship winner Cameron Smith has been linked with joining LIV Golf, and McIlroy said he has spoken to the Australian.
“I don’t care if they leave or not, it won’t change anything for me, but I would like people to at least make an informed decision,” said McIlroy from Northern Ireland.
Speaking ahead of the Tour Championship – the final PGA Tour event of the season – Monahan explained that the top players will play in all four major tournaments, the Players Championship, the 12 events with increased prize money and three additional events per season.
Other changes include an increased payout for the Player Impact Program, which pays players who generate coverage for the tour.
This amount will be doubled from $50m (£42.3m) to $100m (£84.6m) and will reward 20 players.
There will also be guaranteed prize pools of at least $500,000 (£423,000) for every player in the Korn Ferry Tour development priority category and above.
Monahan was also asked if making these changes earlier might have prevented LIV Golf from starting.
“I don’t think there’s a scenario where they weren’t launching,” he replied.
“I think the decisions we are making right now are the right ones for this organization, and we will continue to make more.”