Bryson DeChambeau and seven members of the European Ryder Cup squad will face next year’s Saudi international, putting them on a collision course with their home tours.
The players are among 25 revealed by the organizers of the event, which will be played near Jeddah from February 3-6.
Their participation puts them in conflict with the PGA and DP World Tours, who have indicated that they will not provide releases to their players.
This can result in fines or suspensions for some of the biggest names in the game.
Former US Open winner DeChambeau will be joined by fellow Americans Dustin Johnson, Phil Mickelson, Bubba Watson and Jason Dufner, as well as Olympic champion Xander Sc Chaudele at the Royal Greens Golf and Country Club.
The list also includes Paul Casey, Tommy Fleetwood, Sergio Garcia, Tyrrell Hatton, Shane Lowry, Ian Poulter and Lee Westwood. They were all part of the European Ryder Cup team beaten at Whistling Straits in September.
There is a strategic alliance between the American and European circuits. Both are wary of a possible Saudi super-league that could involve the main global players.
The Saudi February tournament, which was previously part of the European Tour program, is now the flagship event of the Asian Tour.
“The Asian Tour has gone from being a partner to being a competitor,” said European Tour president David Williams in a recent interview with The Scotsman. “And we are fierce competitors.”
The Saudis have named former world number one Greg Norman as their figurehead. As chairman of Liv Golf Investments, the Australian is overseeing an initial $ 200million (£ 140million) pumped into the Asian circuit.
The 66-year-old said “this is just the start” when he was announced as chief executive last month.
“Do I think Saudi Arabia could become a golf powerhouse? Yes, I think so,” Norman added in a recent interview with Arab News.
Other players who have confirmed they will play the $ 5million Saudi event next year include Ryder Cup vice-captains Graeme McDowell and Henrik Stenson as well as former world number one Adam Scott.
Organizers say it will be the strongest international peloton ever assembled on the Asian Tour.
The DP World Tour told BBC Sport they have no comment on the news at this point, but their reaction and that of the PGA Tour will be closely watched by major players around the world.